LOCUS       ISVsa3-tet(X4)-ISVsa3_unit5586 bp   DNA    linear   BCT 19-OCT-2020
DEFINITION  Escherichia coli.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia
            coli.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISVsa3-tet(X4)-ISVsa3_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
     mobile_element  1..5586
                     /gene="ISVsa3-tet(X4)-ISVsa3_unit"
                     /product="Putative resistance unit: ISVsa3-tet(X4)-ISVsa3
                     unit"
     mobile_element  1..977
                     /gene="ISVsa3"
                     /product="Insertion sequence: ISVsa3"
     repeat_region   1..26
                     /gene="oriIS"
                     /product="ISVsa3 oriIS"
     CDS             41..964
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISVsa3 transposase"
                     /translation="MLFLDGVYVEQSHGSARFRWVKAPTSPELTQLTHTIAHRVGRYL
                     ERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKVFTLQTLPTSGD
                     PFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLTRGGNVRYQLK
                     TPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALVTPAKRGRGN
                     KVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIEDPIVIKQIL
                     DHLKHKAETSGTRALPESRAPPAELLLGLFD"
     repeat_region   952..977
                     /gene="terIS"
                     /product="ISVsa3 terIS"
     CDS             complement(1456..1656)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MLVSFTMPANLYIYETFYSISYPYGKIGECFYYFISHFEFLKYK
                     NYFNILVLIVLNYLCFKSYEII"
     CDS             1748..2593
                     /codon_start=1
                     /transl_table=11
                     /product="Alpha/beta hydrolase"
                     /translation="MKEKIIKTNGIELCTESFGNKKNPAILLVAGATVSMLYWDTEFC
                     QQLSEKGFFVIRYDNRDVGKSTNYEPGSAPYDIVDLTNDAIAILDGYEIDKAHFVGMS
                     LGGLITQIAAIKFADRVSSLTLMSTGPWGDSDPTIPEMDTRILDFHGKAGTVDWSNED
                     SVVNYLIQGAELMCGKKQFDKQRSEKLIRAEFNRANNYISMFNHATLQGSEEYWNRLN
                     EVNQPTLIIHGTDDKIWHYKNAGVLIEKIQNTKLITLEGTGHELHFEDWTTIIDGIEK
                     HINEQ"
     CDS             2583..3740
                     /gene="tet(X4)"
                     /codon_start=1
                     /transl_table=11
                     /product="Tetracycline-inactivating monooxygenase"
                     /translation="MSNKEKQMNLLSDKNVAIIGGGPVGLTMAKLLQQNGIDVSVYER
                     DNDREARIFGGTLDLHKGSGQEAMKKAGLLQTYYDLALPMGVNIADEKGNILSTKNVK
                     PENRFDNPEINRNDLRAILLNSLENDTVIWDRKLVMLEPGKKKWTLTFENKPSETADL
                     VIIANGGMSKVRKFVTDTEVEETGTFNIQADIHHPEVNCPGFFQLCNGNRLMAAHQGN
                     LLFANPNNNGALHFGISFKTPDEWKNQTQVDFQNRNSVVDFLLKEFSDWDERYKELIR
                     VTSSFVGLATRIFPLGKSWKSKRPLPITMIGDAAHLMPPFAGQGVNSGLMDALILSDN
                     LTNGKFNSIEEAIENYEQQMFIYGKEAQEESTQNEIEMFKPDFTFQQLLNV"
     mobile_element  4610..5586
                     /gene="ISVsa3"
                     /product="Insertion sequence: ISVsa3"
     repeat_region   4610..4635
                     /gene="oriIS"
                     /product="ISVsa3 oriIS"
     CDS             4650..5573
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISVsa3 transposase"
                     /translation="MLFLDGVYVEQSHGSARFRWVKAPTSPELTQLTHTIAHRVGRYL
                     ERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKVFTLQTLPTSGD
                     PFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLTRGGNVRYQLK
                     TPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALVTPAKRGRGN
                     KVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIEDPIVIKQIL
                     DHLKHKAETSGTRALPESRAPPAELLLGLFD"
     repeat_region   5561..5586
                     /gene="terIS"
                     /product="ISVsa3 terIS"
BASE COUNT     1597 a   1235 c   1433 g   1321 t
ORIGIN
        1 gcgttttgga tcggcgctca atctgaatgt tcacttccac atgctgtttc tcgacggtgt
       61 gtatgtcgag caatcccacg gctcagcgcg tttccgctgg gtcaaggcgc cgaccagccc
      121 agagctcacc cagctgacgc acaccatcgc ccaccgggtg ggtcgctatc tggaacggca
      181 aggcctgctg gaacgggatg tcgaaaacag ctatctggcc tcggatgcgg tggatgacga
      241 cccgatgaca cccctgctgg ggcactcgat cacttaccgt atcgctgtcg gttcacaggc
      301 ggggcgaaag gtgttcactt tgcaaactct gccgaccagt ggtgatccgt tcggtgacgg
      361 gattggcaag gtagccgggt ccagcctgca cgccggcgtg gcggccaggg ccgatgaacg
      421 caagaagctc gaacggctgt gccggtacat cagccgcccg gcggtatccg agaagcggct
      481 gtcgttaaca cgaggcggca acgtgcgcta ccagctcaag acgccgtacc gggacggcac
      541 cacgcacgtc attttcgaac cattggattt cattgcaagg ctggccgccc tggtaccgaa
      601 gcccagagtc aacctaaccc gcttccacgg ggtgttcgca cccaacagtc ggcaccgggc
      661 gttggtcacg ccggcaaaac ggggcagggg caacaaggtc agggtggctg atgaaccggc
      721 aacaccagca caacggcgag cgtcgatgac atgggcgcaa cggctcaagc gtgttttcaa
      781 tatcgacatc gagacctgca gcggctgcgg cggcgccatg aaagtcatcg cctgcattga
      841 agaccctata gtgatcaagc agatccttga tcacctgaag cacaaagccg aaaccagcgg
      901 gaccagggcg ttacccgaaa gccgggcgcc accggctgag ctgctcctgg gtctgtttga
      961 ctgacgagcc tgaaggccaa cgataccaat caaaatgctg cgttcacagc gccgcggcag
     1021 ggatccgccg tgctggttgt cggaaaagga gccgctagtg ggaaagagga gggtaaattt
     1081 tcagcgttgc tggctccccg tcagccggat tgggttgcat cgcaggggtg tcgaaagagt
     1141 caactgcggt ccaaagctgt tggacttggg tgaaaagggc gtttattctt cctatacgtc
     1201 aataggaaat attgatgaaa tttgttgctt aagattgttt gctggtgtct gtaaatcaag
     1261 gttctgagca atagaactct gtgcgatagc aaaggtcgct atcgacatat aaattaattt
     1321 ctttttcatg gattttcttt ttcaaaaccc aagttataaa ttttttacta actataaact
     1381 cctttttctg ttaattttaa attttaacat ttgacacaac tttaatatat ttatatatac
     1441 atatattttt atatattata tgatttcata agatttgaaa cataaataat taagcactat
     1501 aagtactaaa atgttaaaat aatttttata tttgagaaat tcaaaatgcg aaataaaata
     1561 gtaaaaacat tcgcctatct tgccatatgg gtatgaaata gagtagaatg tttcgtatat
     1621 atataagtta gcgggcattg tgaaagatac cagcataaca gttaaacatt gataaatgaa
     1681 aaaaaaacta ctttggatat taattttagg actgataata atcagttgca aacaaaggaa
     1741 aacagaaatg aaagagaaaa taattaaaac aaacggcatt gaactctgta cggaaagttt
     1801 tggaaataag aaaaatccag caatcctttt ggtagcaggt gcaaccgtat caatgctgta
     1861 ttgggacact gaattttgcc aacaattatc tgaaaaagga ttttttgtta ttcgttacga
     1921 caacagagat gtaggaaaat ccactaatta tgagccgggt tcagcaccat acgacattgt
     1981 tgacttaaca aatgatgcca ttgcaatttt ggacggctat gaaatagaca aagcacattt
     2041 tgtgggtatg tcattaggtg ggctaattac acaaatagca gctataaagt ttgccgatag
     2101 agttagctca ttaacactta tgtctacagg cccttgggga gactcggacc caactattcc
     2161 tgagatggac acacgtattt tagatttcca tggtaaagca ggcacagtcg attggtcaaa
     2221 tgaagacagt gtggtaaact atttaattca gggtgcagaa ttaatgtgcg gcaagaaaca
     2281 atttgataaa caaagaagtg aaaagctgat aagagctgag ttcaatagag caaacaatta
     2341 tataagtatg ttcaatcacg ctacattgca aggtagtgaa gaatattgga atagactaaa
     2401 tgaagtaaat caaccaacct tgataattca cggaacagat gacaaaattt ggcactataa
     2461 aaacgctggt gtattaattg agaaaataca aaatacgaaa ttaattactc ttgaaggaac
     2521 aggacacgaa ttgcactttg aagattggac aacaataatt gacggaattg aaaaacatat
     2581 aaatgagcaa taaagaaaaa caaatgaatt tacttagtga taagaacgtt gcaataattg
     2641 gtggtggacc cgttggactg actatggcaa aattattaca gcaaaacggc atagacgttt
     2701 cagtttacga aagagacaac gaccgagagg caagaatttt tggtggaacc cttgacctac
     2761 acaaaggttc aggtcaggaa gcaatgaaaa aagcgggatt gttacaaact tattatgact
     2821 tagccttacc aatgggtgta aatattgctg atgaaaaagg caatatttta tccacaaaaa
     2881 atgtaaagcc cgaaaatcga tttgacaatc ctgaaataaa cagaaatgac ttaagggcta
     2941 tcttgttgaa tagtttagaa aacgacacgg ttatttggga tagaaaactt gttatgcttg
     3001 aacctggtaa gaagaagtgg acactaactt ttgagaataa accgagtgaa acagcagatc
     3061 tggttattat tgccaatggt ggaatgtcta aagtaagaaa atttgttacc gacacggaag
     3121 ttgaagaaac aggtactttc aatatacaag ccgatattca tcatccagag gtgaactgtc
     3181 ctggattttt tcagctatgc aatggaaacc ggctaatggc tgctcatcaa ggtaatttat
     3241 tatttgcgaa tcctaataat aatggtgcat tgcattttgg aataagtttt aaaacacctg
     3301 atgaatggaa aaaccaaacg caggtagatt ttcaaaacag aaatagtgtc gttgattttc
     3361 ttctgaaaga attttccgat tgggacgaac gctacaaaga actgattcgt gtgacatcat
     3421 cttttgtagg gttagcgaca cgaatatttc ccttaggtaa gtcttggaaa agtaagcgtc
     3481 cattacccat aacgatgatt ggagatgctg ctcatttgat gcctcctttt gcaggacaag
     3541 gcgtaaacag cgggttgatg gatgccttga tattgtcgga taatctgacc aatgggaaat
     3601 ttaacagcat tgaagaggct attgaaaatt atgaacagca aatgtttatc tatggcaaag
     3661 aagcacaaga agaatcaact caaaacgaaa ttgaaatgtt taaacccgac tttacgtttc
     3721 agcaattgtt aaatgtataa aatggaataa aacggcacat aatcgagtag acggctccgc
     3781 cagtaactga cggagtgcgc ctctcacacc accgagcgta cgggtcacgt actcggcggt
     3841 tcgcaaagag atgggttaag ttgtaaatgt aattcagtta aggaaacata tcctcttttc
     3901 ttcaggcgtt gtaaagtaat ggtagtgcct agaataggac tttgtgccaa ctattgagtg
     3961 ggagtgacgg gcactggctg gcaatgtcta gcaacggcag gcatttcggc tgagggtaaa
     4021 agaactttcc gctaagcgat agactgtatg taaacacagt attggcaagg acgcggaaca
     4081 tgcctcatgt ggcggccagg acggccagcc gggatcggga tactggtcgt taccagagcc
     4141 accgacccga gcaaaccctt ctctatcaga tcgttgacga gtattacccg gcattcgctg
     4201 cgcttatggc agagcaggga aaggaattgc cgggctatgt gcaacgggaa tttgaagaat
     4261 ttctccaatg cgggcggctg gagcatggct ttctacgggt tcgctgcgag tcttgccacg
     4321 ccgagcacct ggtcgctttc agctgtaagc gtcgcggttt ctgcccgagc tgtggggcgc
     4381 ggcggatggc cgaaagtgcc gccttgctgg ttgatgaagt actgcctgaa caacccatgc
     4441 gtcagtgggt gttgagtttc ccgtttcagc tgcgtttcct gtttgccagc cggcccgaga
     4501 tcatggggtg ggtgctgggc atcgtttacc gcgtcattgc cacgcacctg gtcaagaaag
     4561 cgggccatac ccaccaagtg gccaagacgg gcgcggtcac cctgatccag cgttttggat
     4621 cggcgctcaa tctgaatgtt cacttccaca tgctgtttct cgacggtgtg tatgtcgagc
     4681 aatcccacgg ctcagcgcgt ttccgctggg tcaaggcgcc gaccagccca gagctcaccc
     4741 agctgacgca caccatcgcc caccgggtgg gtcgctatct ggaacggcaa ggcctgctgg
     4801 aacgggatgt cgaaaacagc tatctggcct cggatgcggt ggatgacgac ccgatgacac
     4861 ccctgctggg gcactcgatc acttaccgta tcgctgtcgg ttcacaggcg gggcgaaagg
     4921 tgttcacttt gcaaactctg ccgaccagtg gtgatccgtt cggtgacggg attggcaagg
     4981 tagccgggtc cagcctgcac gccggcgtgg cggccagggc cgatgaacgc aagaagctcg
     5041 aacggctgtg ccggtacatc agccgcccgg cggtatccga gaagcggctg tcgttaacac
     5101 gaggcggcaa cgtgcgctac cagctcaaga cgccgtaccg ggacggcacc acgcacgtca
     5161 ttttcgaacc attggatttc attgcaaggc tggccgccct ggtaccgaag cccagagtca
     5221 acctaacccg cttccacggg gtgttcgcac ccaacagtcg gcaccgggcg ttggtcacgc
     5281 cggcaaaacg gggcaggggc aacaaggtca gggtggctga tgaaccggca acaccagcac
     5341 aacggcgagc gtcgatgaca tgggcgcaac ggctcaagcg tgttttcaat atcgacatcg
     5401 agacctgcag cggctgcggc ggcgccatga aagtcatcgc ctgcattgaa gaccctatag
     5461 tgatcaagca gatccttgat cacctgaagc acaaagccga aaccagcggg accagggcgt
     5521 tacccgaaag ccgggcgcca ccggctgagc tgctcctggg tctgtttgac tgacgagcct
     5581 gaaggc
//
